PREVELANCE AND ASSOCIATED RISK FACTOR OF ESCHERICHIA COLI FROM THE CASES OF POULTRY AT REGIONAL VETERINARY LABORATORY (RVL) SURKHET, NEPAL
1- B.V.Sc& A.H, 17th Batch, IAAS, Rampur campus, Chitwan, Nepal
2-Senior Veterinary Officer, Regional Veterinary Laboratory (RVL), Surkhet, Nepal
A study was conducted at Regional Veterinary Laboratory (RVL), Birendranagar, Surkhet from September 2016 to January 2017 to find out the prevalence and associated risk factor of E.coli from the cases of brought to RVL, Surkhet. Hundred (100) liver samples brought at RVL for postmortem of poultry were collected for the study and questionnaire survey was conducted to take demographic data. Isolation and identification of organisms and were made by examination for cultural characteristics in Nutrient agar, Mac-Conky agar, EMB agar, Gram’s stain and biochemical tests (Indol test(+ve), Mr test(+ve),Vp test(-ve), Citrate utilization test(-ve) and Catalase test(+ve) ). The results showed that the prevalence of E. coli in poultry was found to 31% positive. A total of 31 positive cases were kept for antibiotic sensitivity test against six antimicrobials: Amoxycillin (AMX-10 Mcg), Azithromycin (AZM-15 Mcg), Colistin Sulphate (CL-10mcg), Enrofloxacin (EX-5 Mcg), Gentamycin (GEN-10 Mcg) and Tetracycline (TE-30mcg) .The sensitivity test was done by standard disk diffusion method.
The overall prevalence of E.coli was found to be 31%. On statistical analysis for the risk factors according to age, source of water and frequency of clean drinker was found to be non- significant (P > 0.05) and status of water (treated/untreated) was found to be significant (P < 0.05). The antibiotic sensitivity test on the positive case were found to be: Colistin sulphate-96.77%, Azithromicin,-80.64%, Gentamicin-70.96%, Enrofloxicin-38.71%, Tetracyclin-22.58%, Amoxycilin-0% sensitive and Amoxycilin-100%, Tetracyclin-77.42%, Enrofloxicin-61.29%, Gentamicin-25.80%, Azithromicin-19.35%, Colistin sulphate-3.22% resistant respectively. Out of total 100 cases from Surkhet, 75 poultry farmers used tap water and 25 used deep well as a source of water in which , 4 were obtained from deep well and 27 were obtained from tap water source for drinking purpose in poultry were positive. All the risk factors I had considered are statistically non significant except status of water (treated/un-treated) So, I recommend to provide treated water for the poultry to avoid E.coli contamination and further studies should be done considering other risk factors. Use of antibiotic in poultry is recommended only after scheduled and proper sensitivity analysis.
Key words: Prevalence, E. coli, Sensitivity, Resistance
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